S. tiburo reproduces sexually and is viviparous. Maturity is reached at about 75 cm (30 in). The pups are born in late summer and early fall and measure 30-32 cm (12-13 in) at birth and approximately 172 g. Usually eight to twelve pups are produced in each litter.
Survivorship for young individuals, especially newborn pups, may be affected by size-selective predation.
Geographic variation does seem to have an effect on the survival of the pups, as well as the weight and size. In Florida Bay and Tampa Bay there was a study of two populations of bonnethead sharks. Size at maturation, age at maturation, time of fertilization, rate of embryonic development, size at birth, the energetic investment in producing offspring, gestation period, and the incidence of fertility were found to differ between these population. Food limitations and seasonal differences may play a role in these differences (Castro, 1987).