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Nigrospora oryzae as Fungal Endophyt in Rice (Oryza sativa,L.)

         Nigrospora oryzae is a weak pathogen causing grain spots of ice , sorghum , corn and some species of grasses.  It is generally transmitted vertically through seeds ( Kumar, et. al. 2015). Its strain  Nigrospora oryzae EFTPL1 can be a saphrobe or parasite common in rice and other plants. It is transmitted through seeds and settle on leaves without causing disease to the plants. 

        Nigrospora oryzae has a white surface colony; cream to white reverse surface with small patch of black in the center; mycelia abundant; 40-45 mm diamter after 7 days of incubation at 28 degrees Celsius on Potato Dextrose Agar. 

       The results of molecular identification of Nigrospora oryzae shown on the Internal Transcribed Spacer (ITS) region of ribosomal DNA (rDNA) specifically on ITS-1 and ITS-4 primers revealed a total of 1392 bases and exactly ~534bp 99% matched with ~534bp of Nigrospora oryzae from GenBAnk (Lou, 2014).




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© Joscel Enardecido

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