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Commonly Used Nouns You Should Know About NGS

What is High-throughput Sequencing?

High-throughput sequencing (HTS) is a revolutionary change to traditional Sanger sequencing (known as one-generation sequencing), which involves sequencing hundreds of thousands to millions of nucleic acid molecules at one time. Therefore, it is named next generation sequencing (NGS) in some literature as a landmark change. Meanwhile, high-throughput sequencing makes it possible to analyze the detailed transcriptome and genome of a species, because of which it is also called deep sequencing.

What is Genome Re-sequencing?

Genome-wide sequencing is a method for genomic sequencing of individuals with known genomic sequences and for differential analysis at the individual or population level. As the cost of genome sequencing continues to decline, the study on pathogenic mutations of human diseases has been expanded from the exon region to the whole genome range. It is of significant scientific research and industrial value to detect disease related common, low-frequency and even rare mutations at the whole genome level by construction of different lengths of inserted fragment library as well as short-sequence and double-ended sequencing strategy.

What is De Novo Sequencing?

De novo sequencing is a sequencing method that can sequence a species without any existing sequence data. It can obtain the genome map of the species by sequence splicing and assembly with bioinformatics analysis. Obtaining the genome sequence of a species is an important shortcut to speed up the understanding process of this species. With the rapid development of the next generation sequencing technology, the cost and time of genome sequencing are greatly reduced compared with traditional technology.

What is mRNA Sequencing?

Transcriptomics (transcriptomics) is emerged after Genomics. It aims at studying all RNA types and copies of a particular cell transcribed under a specific functional state, including mRNA and non-coding RNA. Creative Biolabs' mRNA transfection technology enables a variety of related studies and new discoveries throughout the entire mRNA domain. MRNA sequencing is not designed for primers or probes and is free to provide objective and authoritative information about transcription. Researchers only needed a single test to quickly generate complete RNA sequence information for the complete poly-A tail and to analyze gene expression, cSNP, brand new transcripts, novel isomers, splice sites, allele-specific expression as well as rare transcription and the most comprehensive transcriptome information. Simple sample preparation and data analysis software supports mRNA sequencing studies in all species.

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