Summary DescriptionAPI English: Uropod formation in Entamoeba histolytica. Trophozoites were seeded on glass bottom culture dishes (MatTeck) and incubated at 37Â°C in the presence of 5 Âµg/ml of fluorescent Con A (Alexa fluor 488, Molecular Probes). Live parasites undergoing capping were imaged using a confocal microscope (40Ã objective) with a Nipkow disk device (Perkin Elmer). Images (10 per second) in a focal plane show fluorescence changes at the amoebic membrane reflecting the surface receptor capping and uropod formation phenomena. Date April 2011 Source Video S1 from Marquay Markiewicz J, Syan S, Hon C, Weber C, Faust D, Guillen N (2011). "A Proteomic and Cellular Analysis of Uropods in the Pathogen Entamoeba histolytica". PLOS Neglected Tropical Diseases. DOI:10.1371/journal.pntd.0001002. PMID21483708. PMC: 3071361. Author Marquay Markiewicz J, Syan S, Hon C, Weber C, Faust D, Guillen N Permission (Reusing this file) This file is licensed under the Creative CommonsAttribution 2.5 Generic license. You are free: to share â to copy, distribute and transmit the work to remix â to adapt the work Under the following conditions: attribution â You must attribute the work in the manner specified by the author or licensor (but not in any way that suggests that they endorse you or your use of the work). http://creativecommons.org/licenses/by/2.5 CC BY 2.5 Creative Commons Attribution 2.5 truetrue This file was published in a Public Library of Science journal. Their websitestates that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise. Provenance This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.