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A-Proteomic-and-Cellular-Analysis-of-Uropods-in-the-Pathogen-Entamoeba-histolytica-pntd.0001002.s002


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Summary[edit] DescriptionAPI English: Uropod formation in Entamoeba histolytica. Trophozoites were seeded on glass bottom culture dishes (MatTeck) and incubated at 37°C in the presence of 5 µg/ml of fluorescent Con A (Alexa fluor 488, Molecular Probes). Live parasites undergoing capping were imaged using a confocal microscope (40× objective) with a Nipkow disk device (Perkin Elmer). Images (10 per second) in a focal plane show fluorescence changes at the amoebic membrane reflecting the surface receptor capping and uropod formation phenomena. Date April 2011 Source Video S1 from Marquay Markiewicz J, Syan S, Hon C, Weber C, Faust D, Guillen N (2011). "A Proteomic and Cellular Analysis of Uropods in the Pathogen Entamoeba histolytica". PLOS Neglected Tropical Diseases. DOI:10.1371/journal.pntd.0001002. PMID 21483708. PMC: 3071361. Author Marquay Markiewicz J, Syan S, Hon C, Weber C, Faust D, Guillen N Permission
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