Summary DescriptionAPI English: HDAC8 promotes centripetal movement of endosomes containing internalized WGA. Control (All*Neg) and HDAC8-depleted (si HDAC8) A549 cells grown in 96-well Matrix plates were incubated with imaging medium containing WGA-AF647 (5 Âµg/ml). After addition of WGA (tâ=âzero), time-lapse images were acquired with the MD Assay Development 2 microscope at 15 min intervals up to 6 h, using a 20Ã objective. The video plays at 15 fps. Date October 2011 Source Video S1 from Yamauchi Y, Boukari H, Banerjee I, Sbalzarini I, Horvath P, Helenius A (2011). "Histone Deacetylase 8 Is Required for Centrosome Cohesion and Influenza A Virus Entry". PLOS Pathogens. DOI:10.1371/journal.ppat.1002316. PMID22046129. PMC: 3203190. Author Yamauchi Y, Boukari H, Banerjee I, Sbalzarini I, Horvath P, Helenius A Permission (Reusing this file) This file is licensed under the Creative CommonsAttribution 2.5 Generic license. You are free: to share â to copy, distribute and transmit the work to remix â to adapt the work Under the following conditions: attribution â You must attribute the work in the manner specified by the author or licensor (but not in any way that suggests that they endorse you or your use of the work). http://creativecommons.org/licenses/by/2.5 CC BY 2.5 Creative Commons Attribution 2.5 truetrue This file was published in a Public Library of Science journal. Their websitestates that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise. Provenance This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.